Journal: Genetics in medicine : official journal of the American College of Medical Genetics

Article Title: Neurofilament Light Chain Levels Correlate with Clinical Measures in CLN3 Disease

doi: 10.1038/s41436-020-01035-3

Figure Lengend Snippet: CSF and serum sample characteristics and assayed methods.

Article Snippet: We used a solid-phase sandwich ELISA kit (UmanDiagnostics, Umea, Sweden; Catalog number: 10-7002 RUO) and followed the manufacturer’s instructions to measure CSF NEFL levels.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Genetics in medicine : official journal of the American College of Medical Genetics

Article Title: Neurofilament Light Chain Levels Correlate with Clinical Measures in CLN3 Disease

doi: 10.1038/s41436-020-01035-3

Figure Lengend Snippet: CSF NEFL level in samples from CLN3 versus non-CLN3 (PLC, CTD, SLOS) individuals, measured by ELISA. Black diamond – mean; blue diamond – outlier; box – median and interquartile range [IQR, Q1 (25 th percentile) - Q3 (75 th percentile)]; whiskers – minimum = Q1 – 1.5*IQR and maximum = Q3 + 1.5*IQR. P values are derived from ANOVA and post-hoc Bonferroni adjustment for pairwise multiple comparisons. CSF: cerebrospinal fluid. CTD: creatine transport deficiency. NEFL: neurofilament light chain. PLC: pediatric laboratory controls. SLOS: Smith-Lemli-Opitz syndrome.

Article Snippet: We used a solid-phase sandwich ELISA kit (UmanDiagnostics, Umea, Sweden; Catalog number: 10-7002 RUO) and followed the manufacturer’s instructions to measure CSF NEFL levels.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Molecular Medicine Reports

Article Title: Understanding the role of iron/heme metabolism in the anti‑inflammatory effects of natural sulfur molecules against lipopolysaccharide‑induced inflammation

doi: 10.3892/mmr.2025.13542

Figure Lengend Snippet: Sulfur compounds inhibit LPS-induced inflammation and iron/heme metabolism. (A) Flow cytometry showing Fe2+ levels in THP-1 cells following treatment with LPS (10 ng/ml) + NTS (3 µg/ml), MSM (200 mM) or TLR-C34 (40 µM) for 48 h. Western blot analysis of (B) transferrin receptor, ferroportin, DMT1 and STEAP3; and (C) MFRN, ABCB6, ABCB10, ALAS1, HO-1, FECH and FLVCR proteins in THP-1 cells following treatment with LPS (10 ng/ml) + NTS (3 µg/ml), MSM (200 mM) or TLR-C34 (40 µM) for 48 h. ALAS1, 5′-aminolevulinate synthase 1; DMT1, divalent metal transporter 1; Fe2+, ferrous ion; FECH, ferrochelatase; FPN, ferroportin; HO-1, heme oxygenase-1; LPS, lipopolysaccharide; MFRN, mitoferrin; MSM, methylsulfonylmethane; NTS, nontoxic sulfur; TfR, transferrin receptor; TLR, Toll-like receptor.

Article Snippet: The following primary antibodies were purchased from Santa Cruz Biotechnology, Inc.: Ferrochelatase (FECH; cat. no. sc-377377), phosphorylated (p)-IκBα (cat. no. sc-8404), TLR2 (cat. no. sc-21759), TLR4 (cat. no. sc-293072), COX-1 (cat. no. sc-19998), COX-2 (cat. no. sc-19999, IKKα/β (cat. no. sc-7607), and β-actin (cat. no. sc-47778); from Cell Signaling Technology, Inc.: IL-1β (cat. no. 12703), p-ERK (cat. no. 9101), ERK (cat. no. 9102), IκBα (cat. no. 9242), p-IKKα/β (cat. no. 2697), NF-κB (cat. no. 8242), p-p38 (cat. no. 4511), p38 (cat. no. 8690), ATR (cat. no. 2790), ATM (cat. no. 2873), Chk2 (cat. no. 2662), BRCA1 (cat. no. 9010), p53 (cat. no. 9282), p-MDM2 (cat. no. 3521), MDM2 (cat. no. 86934) and the DNA Damage Antibody Sampler Kit (cat. no. 9947; containing p-ATM, p-ATR, p-Chk2, p-BRCA1 and p-p53 antibodies); from Abcam: Divalent metal transporter (DMT)1 (cat. no. ab55735), 5′-aminolevulinate synthase (ALAS)1 (cat. no. ab84962), STEAP3 (cat. no. ab151566), HO-1 (cat. no. ab137749), transferrin receptor (TfR; cat. no. ab84036), PKCα (cat. no. ab179523), p-PKCα (cat. no. ab59411), TNF-α (cat. no. ab183218), IL-6 (cat. no. ab6672) and TATA-binding protein (TBP; cat. no. ab818); from LifeSpan BioSciences, Inc.: ABCB10 (cat. no. LS-C381841) and FLVCR1 (cat. no. LS-C750126); from LS Bio; Vector Laboratories, Inc.: FPN (cat. no. NBP1-21502) and iNOS (cat. no. NB300-605); from Novus Biologicals; Bio-Techne: Mitoferrin (MFRN; cat. no. MBS6013473); and from Boster Biological Technology: ABCB6 (cat. no. PA1723).

Techniques: Flow Cytometry, Western Blot